【摘要】 利用含1106条水稻独立基因的cDNA微阵列,检测水稻近等基因系H7R/H7S受稻瘟病菌诱导8h的表达谱差异。结果显示:基因表达谱变化明显,在980组有效数据中,共检测到170条基因的表达丰度发生显著变化,其中上调表达基因106条,下调表达基因64条。通过与NCBI水稻数据库进行BLASTn比对分析,发现这些差异表达基因包含β-1,3-葡聚糖酶、富甘氨酸蛋白在内的已知基因33条,未知基因137条。对10个未知基因进行生物信息学分析后,其中获得一个含全长基因并具有BTB/POZ结构域的克隆T007F02(http://www.estarray.org),该基因命名为OsBTB。该克隆的cDNA序列具有完整的ORF区,OsBTB上游具有典型的启动子区。结果经测序得到证实。高通量的RNA斑点杂交证实:在抗性供试水稻中,该基因在水稻接种稻瘟病菌早期(8~12h)受诱导高表达;在感病品种中,该基因表达无明显改变。结果提示OsBTB基因在功能上可能与水稻抗瘟性过程密切相关。更多还原

【Abstract】 cDNA arrays comprising of 1 106 uni-genes were used to monitor the expression profile of rice genes of near-isogenic line H7R/H7S 8 h after the infection by Magnaporthe grisea. The results showed that expression profile of rice genes changed remarkably. Of the 980 genes which were available in the cDNA arrays, 106 genes were up-regulated and 64 genes were down-regulated by at least two-fold. BLAST analysis against GenBank rice database revealed that 33 genes are homologous to known genes, including β-1,3-glucanase and glycine-rich protein genes. By bioinformatics analysis to 10 unknown genes, a clone, T007F02, contained a full-length new gene with BTB/POZ domain (http://www.estarray.org) was defined and sequenced, which was now named as OsBTB. The analysis results of RNA blot indicated that OsBTB was highly expressed in early stage of resistant rice samples inoculated by pathogen, and not changed in susceptible varieties. These results implied that OsBTB might play an important role in disease resistance to rice blast.更多还原