【摘要】 根据禾谷镰孢菌参考菌株NRRL31084(PH- 1)的γ微管蛋白基因核苷酸序列设计5对引物,采用PCR方法从禾谷镰孢菌(Fusariumgraminearum)对多菌灵(MBC)的敏感菌株、室内诱导及田间多菌灵抗药性菌株中分段扩增测序,获得了γ- 微管蛋白基因全序列。该基因全长1 868bp,含有5个内元,编码一含493aa的多肽;与PH 1的γ微管蛋白基因核苷酸序列同源性99%,存在10个差异核苷酸,与所编码的氨基酸序列同源性100%;与其它7种真菌的γ微管蛋白基因所编码的氨基酸序列同源性为31% ~72%。中国的2个敏感菌株和4个抗药菌株的γ微管蛋白基因序列完全相同,认为多菌灵抗药性与该微管蛋白变异无关。更多还原

【Abstract】 Fragments of γ-tubulin genes from wild-type of carbendazim(MBC)-sensitive, field and induced MBC-resistant mutants of F. graminearum were amplified, sequenced and spliced into the whole sequence of γ-tubulin gene with 5 pairs of primers synthesized in accordance with the γ-tubulin gene of the reference isolate, NRRL31084(PH-1) by polymerase chain reaction (PCR). γ-tubulin gene had 1 868 bp length, including 5 introns, encoding 493 amino acids. The homology of deduced amino acid sequence of the F. graminearum γ-tubulin gene with that of the other 7 species fungi γ-tubulin genes was from 31% to 72%. The whole nucleotide sequence comparison indicated that there was 10-nucleotide difference between Chinese isolates and PH-1, but with 100% predicted amino acid sequence homology. Sequence comparison among MBC-sensitive, field and induced MBC-resistant isolates revealed there was no mutation, even one.更多还原