【摘要】 枯草芽孢杆菌B9601 -Y2 抑菌蛋白可以使棉花红腐病原菌菌丝畸形,呈念珠状,胞壁穿孔,不规则消解,内含物外泄,失活。B9601- Y2 菌株产抑菌蛋白的最适培养条件为:pH 7 0, 37℃培养 48h。菌株的马铃薯蔗糖培养液经 (NH4 )2SO4 至80%饱和度盐析能得到 0 1mg/mL以上的粗蛋白,粗蛋白的产量与培养过程中供氧条件、产菌量呈正相关。粗蛋白能耐80℃高温和pH9 0的碱性,在 4℃下,抑菌活性 60d内基本不变。更多还原

【Abstract】 Antifungal protein produced by Bacillus subtilis B9601-Y 2 led to deformation,bread string-like,perforation,irregular decomposition,protoplasm leakage and death of Fusarium moniliforme (causing fusarium rot of cotton) hyphae. The optimal condition for the protein yield was to grow in potato sugar juice at pH 7.0 and 37℃ for 48 h,in which crude protein could be obtained about 0.1 mg/mL with ammonium sulphate at 80% saturation. The protein yield was positively related to air volume and the bacterial population. The protein could tolerate 80℃ and pH 9.0 and keep its antifungal activity stable for 60 days at 4℃.更多还原