【摘要】 目的通过合成所有细菌共有的16SrRNA基因高度保守区引物,进行PCR扩增,对8种标准菌株、10种40株临床分离细菌进行了研究,探索快速而准确诊断败血症的新方法,以达到早期治疗的目的。方法通过PCR技术,对40例败血症患者进行了分析,完成了对败血症患者的早期诊断。结果8种标准菌株、10种40株临床分离细菌均获得308bp扩增产物。结论PCR检测方法是一种易于推广的败血症早期诊断方法。更多还原

【Abstract】 ObjectiveIn order to detect the common pathogenic bacteria quickly and accurately, a rapid experimental procedure based upon PCR technology has been set up. MethodsThe first step is the DNA isolation. The DNA was isolated from bacteria by the lysozyme-sodium dodelylsulfate(SDS)-proteinase K-phenol-chloroform method. The second step is the universal 16SrDNA amplification. ResultsAll of the DNAs from 10 kinds of species were amplified well and PCR product which is 308bp was fluorescently labeled by random primer with Cy5-dUTP. obtained. ConclusionPCR technology is an extremely powerful detection system. It can detect the common pathogenic bacteria quickly and accurately. The overall time for sample process lasts about 3 hours. We have demonstrated the sensitivity of detection with PCR technology with as low as 1pg of purified genomic DNA.更多还原