【摘要】 目的　研究血清层粘连蛋白(Laminin ,LN)与宫颈上皮内瘤样病变(CIN)及宫颈癌的关系,并进一步探讨手术和放疗两种治疗方法对血清层粘连蛋白的影响。方法　用酶联免疫吸附试验(enzymelinkedimmunosorbentassay ,ELISA)方法测定11例健康妇女和6 2例宫颈病变患者血清LN含量。结果　CINⅠⅡ级病人血清LN值与正常健康妇女血清LN值无显著差异(P >0 .10 ) ;CINⅢ级病人及宫颈浸润癌患者治疗前血清LN值显著高于正常组(P <0. 0 5 )。经过手术或放疗治疗后其血清LN值显著下降(P <0 . 0 5 ) ;宫颈浸润癌患者其血清LN值与其临床病理期别(F =3 0 2<F0 . 0 5( 2 ,50 ) ,P >0 . 0 5 )及有无淋巴结转移(P >0. 10 )无关而与癌变细胞组织学分化程度有关,即随分化程度降低其血清LN值有升高的趋势。结论　血清LN值可以作为宫颈癌早期诊断指标,并作为肿瘤细胞分化程度的参考。更多还原

【Abstract】 Objective To investigate the relationship between the consistency of laminin in women serum and cervical intraepithelial neoplasia(CIN) cervical carcinoma and to study the effect of surgy and radiotherapy in the consistency of laminin in serum of patients with malignant cervical tumor. Methods Venous blood was drawn from 11 normal women(control group) and 62 cases of women with CIN or cervical carcinoma(study group). We collected venous blood before and 1 week after the operation or radiotherapy, and determined consistency of laminin in serum by enzyme linked immunosorbent assay(ELISA). Results There were not significant differences between the patients with CIN Ⅰ-Ⅱ and the control group( P >0.10); There were significant differences between the patients(before the operation) with CIN Ⅲ; malignant cervical tumor and the control group( P <0.05). The serum laminin consistency of the patients in the study group went down after the therapy( P <0.05). The serum laminin consistency of the patients with malignant cervical tumor was not clearly related to the tumor’s clinical pathological stage and lymph nodes metastasis but related to the degree of histological differentiation of the tumor, that means there was more laminin in the patients with poor-differentiation carcinoma than with well-differentiation carcinoma. Conclusion The serum laminin consistency may be a diagnostic index of malignant cervical tumor and an index of the degree of differentiation of carcinoma cells.更多还原