【摘要】 用不同基因型 ( Ⅳ、Ⅵb、Ⅵg、Ⅶd ( 鸡源 ) 、Ⅶd ( 鹅源 ) 、Ⅷ、Ⅸ ) 新城疫病毒 ( NDV ) 强毒株分别人工感染 2 3 日龄隆昌鹅 , 观察了其对鹅的致病性。结果 : 各株病毒均可使接种鹅感染、发病 , 发病率为 1 0 % ~ 1 0 0 % , 死亡率分别为 0 % 、 2 0 % 、3 0 % 、 7 0 % 、 5 0 % 、 4 0 % 、 5 0 % 。接种Ⅵg、Ⅶd、Ⅷ和Ⅸ型NDV强毒株可使鹅严重发病、死亡 , 症状和病变都与Ⅶd亚型鹅源毒株感染鹅相似 , 接种Ⅳ型 ( Herts 3 3 ) 及Ⅵb亚型 ( PB 9 6 0 1 ) 毒株的鹅轻微发病。免疫组化检测发现 , 攻毒鹅多种组织器官中都能检测到病毒抗原。在能检测到病毒抗原的器官中 , 病毒抗原主要定位于淋巴细胞、网状细胞、巨噬细胞、肝细胞及各种上皮细胞的胞浆内 ; 与Ⅵg、Ⅶd、Ⅷ和Ⅸ型NDV感染鹅组织相比 , Herts 3 3 、PB 9 6 0 1 感染鹅大部分组织内的阳性染色较弱。本试验结果表明 , 不同基因型NDV强毒株对鹅的致病性存在明显差异。更多还原

【Abstract】 The present sutdy investigated the pathogenicity of velogenic ND viruses of 5 different genotypes in 23-day-old goslings. The challenges of these viruses caused 10%-100% morbidity, and 0%-70% mortality in the infected geese, while the control birds remained healthy. While four NDV field isolates and reference strain of chicken origin which fell into genotype Ⅵg, Ⅶd, Ⅷ and Ⅸ, respectively, caused severe clinical disease in geese, and led to substantial death, similar to those of naturally or experimentally diseased animals infected with isolate of goose origin-genotype Ⅶd; geese inoculated with strain Herts/33 (genotype Ⅳ) and isolate PB9601(Ⅵb) exhibited no overt disease, though depression and diarrhea was observed in very few cases. The localization and distribution of the viral antigen in tissues from the infected goslings were investigated by using immunohistochemical(IHC) staining for detection of NDV F protein. The viral antigen could detected frequently in 17 organs and tissues. Specific staining was localized in the cytoplasma of lymphocytes, reticulocytes, macrophages, hepatocytes as well as various epithelial cells. The positive staining was less intensive in several tissues of goslings infected with Herts/33 and PB9601, compared to that from the same tissue of goslings inoculated with genotype Ⅵg, Ⅶd, Ⅷ and Ⅸ, indicative of the differences of the histotropism of different genotypes of ND viruses. The results of the present study showed that different genotypes of velogenic ND viruses have different pathogenicity to geese.更多还原