【摘要】 根据国外已发表的新城疫病毒 F基因序列 ,设计了 1对引物 ,并以 RT- PCR特异性扩增出新城疫病毒 He B0 2分离株的 F基因 ,基因产物大小为 1.6 3kb,与设计相符。对其进行序列测定后 ,与其他标准毒株 F4 8E9、L a Sota和Clone30的 F基因进行了同源性比较 ,结果表明 ,He B0 2株与国内标准强毒株 F4 8E9及目前广泛应用的弱毒疫苗株L a Sota和 Clone30的 F基因核苷酸序列的同源性分别为 88.1%、84 .9%和 83.8%。由此可以看出 ,He B0 2分离株与标准毒株和疫苗株相比 ,在 F基因上发生了变异更多还原

【Abstract】 One pair of primers for amplifying the F gene of NDV were designed, with which the predicted 1.63 kb fragment had been amplified via RT-PCR from NDV strain HeB02. The F gene of strain HeB02 was sequenced and compared with standard strains such as F₄₈E₉, LaSota and Clone30. The results showed that the homologies of nucleic acids between HeB02 strain and F₄₈E₉, LaSota and Clone30 strains were 88.1%, 84.9% and 83.8%, respectively. The difference of the F gene of NDV strain HeB02 with those of other NDV standard strains had been demonstrated.更多还原