【摘要】 【目的】研究大鼠局灶性脑缺血不同缺血时间和不同再灌注时间的脑梗死体积比、皮质半影区脑型葡萄糖转运体(GLUT1、GLUT3)转录水平和蛋白水平的表达。【方法】用线栓法复制大鼠局灶性脑缺血模型,用KontronIBAS2.5全自动图像分析系统检测脑梗死体积比;剥取缺血半影区皮质组织,采用逆转录-聚合酶链反应(RT-PCR),测定GLUT1、GLUT3mRNA水平的变化;用免疫组织化学半定量测定GLUT1、GLUT3蛋白水平的变化。【结果】脑缺血1h后再灌注(MCAO1h/R)较缺血3h再灌注(MCAO3h/R)脑梗死体积明显减小。MCAO1h/R组GLUT1、GLUT3mRNA缺血后升高,24h到达高峰,但GLUT1比GLUT3提前升高,且升高的幅度更大。MCAO3h/R组GLUT1在3h开始升高,24h到高峰;GLUT3在缺血3h有一下降点,然后升高,24h到高峰,一周恢复正常,同样GLUT1比GLUT3提前升高,且升高的幅度更大。MCAO3h/R组较MCAO1h/R组的GLUT1、GLUT3峰值低。GLUT1、3蛋白水平的表达与mRNA相符合。【结论】GLUT1、GLUT3在缺血半影区的表达上调,可能是机体对缺血/再灌注损伤的保护性反应。更多还原

【Abstract】 【Objective】 To investigate the volume percentage of infarct and transcription of brain-type glucose transporters (GLUT1, GLUT3) and the expression of GLUT1, GLUT3 protein at different ischemic time and different reperfusion time in rats’ focal cerebral ischemic penumbra. 【Methods】 Focal ischemic models of middle artery occlusion (MCAO) in rats were made by insertion of nylon thread. Brain samples were harvested from ischemicpenumbra. Infarct volume was analyzed quantitively by Kontron IBAS 2.5 image auto-analyses system. The change ofGLUT1 and GLUT3 mRNA was assessed by RT-PCR. The expression of GLUT1 and GLUT3 protein were assessedby immunohistochemistry.【Results】The infarction volume in MCAO1h/R group was obviously smaller than that inMCAO3h/R group. GLUT1 and GLUT3 began to increase after infarction in MCAO1h/R group, climbed to climax in24 hours. But GLUT1 increased ahead of GLUT3 and the increasing range of the former was greater than that of thelatter. In contrast, in the MCAO3h/R group, GLUT1 began to increase in 3 hours, climbed to climax in 24 hours.GLUT3 began to ascend in 3 hours in MCAO1h/R group, reached to climax in 24 hours and remained higher thannormal in 1 week. Meanwhile, GLUT1 increased ahead of GLUT3 and the increasing range of former was greaterthan that of the latter. The peak value of GLUT1 and GLUT3 in MCAO3h/R group were lower than that in MCAO1h/R. The expression levels of GLUT1 and GLUT3 protein were consistent with the correspoding mRNA.【Conclusions】GLUT1 and GLUT3 expression were notably up-regulated in the penumbra region after focal cerebral ischemia; it may be a protective reaction against ischemic injury.更多还原