【摘要】 目的 :评价镍钛记忆合金表面不同方法处理后的生物相容性。方法 :镍钛形态记忆合金表面氮化钛、类金刚石处理后 ,选择不同方法表面处理试验片 6 0个 (10mm× 5mm× 1mm) ,与骨髓间充质干细胞体外共同培养 ,观察1d、5d、8d培养液中镍离子的释放量、培养液中的羟脯氨酸和碱性磷酸酶含量 ,观察细胞生长情况 ,并进行组织免疫学染色观察细胞内蛋白质含量。结果 :细胞计数第 1d各组间无明显差异 ,第 5d、8d类金刚石及氮化钛组细胞数较镍钛合金组明显增多 (P <0 0 5 )。镍离子释放浓度氮化钛组、类金刚石组与与镍钛记忆合金组之间有差异 (P <0 0 1)。细胞内嗜银蛋白染色光镜下观察类金刚石及氮化钛组核内黑色颗粒较镍钛合金组多并且颗粒较大 ,与镍钛合金组有显著性差异 (p <0 0 5 )。镍钛合金组与类金刚石组细胞培养液中羟脯氨酸含量在第 5d、8d明显较第 1d降低 ,但氮化钛组羟脯氨酸含量逐渐增高 ,与其他两组比较有差异 (p<0 0 5 )。第 5d、8d碱性磷酸酶水平明显下降 ,但氮化钛组与其他组比较有统计学差异 (p <0 0 5 )。第 1d、5d、8d的细胞爬片行转化生长因子 β1免疫组织化学染色光镜下细胞胞浆出现棕色颗粒 ,图像分析仪测灰度值 ,各组间无明显差异 (p >0 0 5 )。结论 :氮化钛表面处理后镍钛记忆合金?更多还原

【Abstract】 Objective To evaluate the biocompatibility of nickel\|titanium shape memory alloy,DLC,TiN surface modification with bone marrow mesenchymal stem cell (BMMSCs)culture in vitro .Methods 60 experimental dises were selected(size 10mm×5mm×1mm)in the study,and BMMSCs were incubated with the test discs. Samples from the cell culture media were collected for determination of nickel,hydroxyproline and hydroxyproline contents on 1,5,8 days Intracellular protein content were observed by immunohistochemical staining method.Results The proliferation of BMMSCs in each group did not show significant difference on 1 day,the cell count have significant differences of cell counting comparing DLC and TiN group with nitinol group on 5 and 8 days ( P <0 05). The nucleus black particles of DLC and TiN groups were larger than the one of nickel group under light microscope by intracellular argentaffin protein staining,there was a significant difference among then ( P <0 05).Release of nickel into cell culture medium in TiN group was significantly decreased than other groups( p <0 01).The hydroxyproline level of nitinol group and DLC group decreased significantly on the fifth,eighth day than on the first day,but the hydroxyprolime of TiN group increased gradually on 1,5,8 days,there were significantly differences between TiN group to the other two groups( p <0 05).The level of the alkaline phosphatase in three groups were lower on the fifth,eighth day than on the first day,and there were significantly difference between TiN group to the other two groups(f p <0 01).The cytoplasmic brown particles were observed on slides by immunohistochemical staining,and the gray scales measured on 1,5,8 days by image analytical tool.TGF\|β 1 expressions didn’t exhibit significant difference among the three groups respectively( p >0 05).Conclusion TiN coating on nickel\|titanium shape memory alloy showed good biocompatibility with bone marrow mesenchymal stem cells in vitro, and potential for clinical usage.更多还原