【摘要】 通过筛选获得对小麦纹枯病有明显防治效果的蜡状芽孢杆菌B946菌株。采用链霉素和利福平抗性标记B946菌株,用平板菌落计数法检测其在小麦根、茎基部、叶内的定殖情况。结果表明:叶部接种B946,该菌能在接种叶内定殖,并能向茎基部、其它叶和根内转移;用B946菌悬液浸种处理,该菌能向茎基部和叶内转移;在一定范围内,菌悬液浓度108cfu/ml以上,浸种时间3h以上,栽培温度25℃,有利于B946在小麦体内的定殖转移。更多还原

【Abstract】 B946 (Bacillus cereus), a strain of the beneficial endophytic bacteria isolated from wheat, could prevent sharp eyespot of wheat. After it was marked with streptomycin and rifampin, colonization of the strain sprayed onto leaves in root, stem-base and leaf of wheat was investigated through diluting method. The results showed that the target strain could colonize internal leaf tissues inoculated by B946 and transfer to internal stem-base, the other leaves and root. And it could also transfer to stem-base and leaf of wheat when seeds were dipped. Higher concentration than 10~8 cfu/ml, 3h for seed treatment, and plant-growing temperature at 25℃ were good for the colonization and transference.更多还原