【摘要】 目的探讨肾上腺髓质素(ADM)与脑缺血再灌注损伤的关系。方法采用线栓法制成大鼠大脑中动脉缺血再灌注模型,阻断血流2 H进行再灌注。应用免疫组织化学法和RT-PCR法检测不同时间段大鼠局灶性脑缺血再灌注后大脑皮质ADM及其MRNA的表达,并进行动态观察。结果正常大鼠大脑皮质有ADM及其MRNA 的表达,假手术组ADM及其MRNA表达略高于正常组,P>0．05;大鼠脑缺血再灌注后大脑皮质ADM及其 MRNA过表达,与正常对照组及假手术组相比差异显著,P<0．05。动态观察发现,脑缺血2 H再灌注2 H,大脑皮质ADM及其MRNA即表达,再灌注22 H达高峰,至1 W仍明显多于正常对照组,P<0．05。结论脑缺血再灌注后ADM及其MRNA呈现规律性过表达。更多还原

【Abstract】 Objective To discuss the relationship between ADM and the injury of focal cerebral ischemia/reperfusion. Methods Construct the model of the rat middle cerebral artery ischemia/reperfusion by the line-block-method which the reperfusion is applied after the ischemia lasting for 2 h. By the method of immunohistochemistry and RT-PCR, we test the expression of ADM and ADM mRNA after focal cerebral ischemia/reperfusion in the brain of rats in different time, and make a dynamic observation at the same time. Results ADM and ADM mRNA express in the normal rat’s brain, but lower than the sham operated group, P >0. 05. There is a over expression of ADM and ADM mRNA after focal cerebral ischemia/reperfusion in the brain of rats, the difference is obviously compared with the normal group and the sham operated group,P<0. 05. After the ischemia last for 2 h, the dynamic observation showed that ADM and ADM mRNA began to express in the cerebral cortex when the reperfusion last for 2 h after, and reach the peak time at 22 h, and still more than the normal group at the time of 1 w, P<0. 05. Conclusion ADM and ADM mRNA showed a regularitily over expression after ischemia/reperfusion of the brain.更多还原