【摘要】 目的检测小鼠脑海马组织中HIF-1α在急性重复缺氧条件下的变化,探讨缺氧预适应过程中HIF-1α活化对脑的保护作用。方法小鼠缺氧0次(HO),1次(H1),4次(H4)后取海马组织,应用RT-PCR技术、Western Blot、EMSA对HIF-1αmRNA、蛋白质含量及HIF-1DNA结合活性进行测定。结果与H0组相比,H1组HIF-1αmRNA显著升高,而H0和H4之间未见显著差异。蛋白质含量及HIF-1DNA结合活性在H0、H1、H4中依次增加。结论HIF-1α蛋白质的含量及HIF-1DNA结合活性增加可能参与缺氧预适应小鼠脑保护。更多还原

【Abstract】 Objective To detect the effect of hypoxia preconditioning on HIF-1α expression in the hippocampus of mice during acute and repeated hypoxia exposures. Methods Mice were randomly divided into 3 groups and were exposed to hypoxia for 4 runs (H4 group) , 1 run (H1 group) and 0 ran (control group). RT-PCR, Western blot and EM-SA were used to examine the HIF-1α expression in the mouse hippocampus following exposure to hypoxia. Total mRNA, total protein and nuclear protein were extracted from the mouse hippocampus for RT-PCR, Western blot and EMSA respectively. Results HIF-1α mRNA levels were found to be increased in H1 and decreased in H4 (vs H1). HIF-1α protein levels and HIF-1 DNA binding activities were increased in H1 and markedly increased in H4. Conclusion HIF-1 activation may be involved in the protection of the brain of hypoxia-preconditioned mice.更多还原