【摘要】 目的观察胰岛素治疗对糖尿病大鼠学习记忆功能障碍及大脑皮层、海马内生长抑素(SS)、血管活性肠肽(VIP)含量变化的作用。方法采用链脲菌素制备雄性Wistar大鼠糖尿病模型,设正常对照组、胰岛素治疗糖尿病组、未治疗糖尿病组。3个月后采用T型水迷宫试验测定大鼠学习记忆功能,采用放免法测定皮层额叶、海马区脑组织SS、VIP含量。结果与正常对照组比较,未治疗糖尿病大鼠游迷宫时间显著延长、正确次数显著减少,额叶皮层、海马区脑组织SS含量显著降低,VIP含量无显著变化;胰岛素治疗组大鼠上述变化无显著意义。结论糖尿病引起大鼠学习记忆功能障碍和SS下降;SS下降与学习记忆功能障碍有关;早期胰岛素治疗可使学习记忆功能和SS含量恢复正常。更多还原

【Abstract】 Objective To study the effects of insulin therapy on alterations of learning and memory functions and somatostatin (SS) and vasoactive intestinal peptide (VIP) levels in frontal cortex and hippocampus of diabetic rats. Methods The diabetic rat models were produced by injection with 1% streptozotocin, followed by insulin administration. The water maze test was used to verify the changes of learning and memory functions of the rats and radioimmunoassary was carried out to observe the alterations of SS and VIP levels in the cerebral cotex and hippocampus. Results The swimming time of untreated diabetic rats in water maze was prolonged significantly and the successful frequencies decreased significantly in comparison to those of controls. The contents of SS reduced significantly both in the frontal cortex and hippocampus of untreated diabetic rats, while VIP were not changed. The changes in learning and memory functions and SS levels in insulin-treated diabetics were not significantly different from those of controls. Conclusion The cognitive dysfunctions induced by diabetes may be correlated with the decrease of SS and alleviated with insulin administration.更多还原