【摘要】 目的 :观察石榴叶鞣质对高脂模型鼠脂代谢的影响 ,以及体外对HMG CoA还原酶的抑制作用。方法 :造大鼠高脂模型 ,以四氧嘧啶诱导高血糖伴高血脂小鼠模型 ,检测TC、TG等生化指标 ;制备大鼠肝微粒体HMG CoA还原酶液 ,测 1min内A34 0nm的变化。结果 :石榴叶鞣质大中剂量 (15 0、75mg kg)对高脂大鼠的TC和TG具有显著的降低作用 ,大小剂量 (30 0、75mg kg)对高血糖伴高血脂小鼠的TC降低作用明显 ,体外抑制HMG CoA还原酶的最低有效浓度为 1× 10 - 6 g mL ,抑制率 15 16 %。结论 :石榴叶鞣质对高脂模型鼠脂代谢紊乱具有较好的调节作用 ,体外可较强的抑制HMG CoA还原酶活性。更多还原

【Abstract】 Objective:To explore the effect of pomegranate leaf tannide (PLT) on lipid metabolite of high lipid rats and its inhibition on HMG CoA reductase in vitro .Methods:The high lipid rat model and high lipid wiih high sugar mice model induced by intraperitoneal injection of alloxan were used.The contents of TC and TG of both models was measured.Rat liver microsome HMG CoA reductase was prepare to measure the content changes of A 340 in 1minute.Results:PLT can remarkably decrease TC and TG of high lipid rat at doses of 150 and 75mg/kg,and its reducing effect on TC of high lipid with high sugar mice at doses of 300 and 75mg/kg is also obvious.The minimal inhibition concentration of HMG CoA reductase in vitro is 1×10 -6 g/mL with inhibition percentage of 15 16%.Conclusion: PLT has a noticeable activity of modulating lipid metabolite of high lipid rat and inhibiting HMG CoA redutase in vitro .更多还原