【摘要】 目的探讨白黎芦醇对人皮肤瘢痕组织成纤维细胞增殖及Ⅰ、Ⅲ型胶原合成及降解影响的机理。方法用MTT法检测及原位杂交技术,对增生性瘢痕用白黎芦醇作用不同时间后,成纤维细胞的增殖及Ⅰ、Ⅲ型前胶原mRNA的原位表达进行了研究。结果(1)白黎芦醇对人皮肤瘢痕组织成纤维细胞的抑制率随浓度的增加和作用时间的延长而增强,最大抑制率可达到93.69%。(2)Ⅰ、Ⅲ型前胶原mRNA 3 d已被明显抑制(P<0.01),7 d后表达强度进一步下降,白黎芦醇对Ⅰ型前胶原mRNA的抑制作用要比Ⅲ型前胶原强。结论(1)白黎芦醇对人皮肤瘢痕组织成纤维细胞增殖有抑制作用,且呈剂量时间依赖性。(2)白黎芦醇对Ⅰ、Ⅲ型前胶原mRNA有明显抑制作用,对Ⅰ型前胶原mRNA的抑制作用要比Ⅲ型前胶原强。更多还原

【Abstract】 Objective To study the effects of Resveratraol on viability of fibroblasts and type Ⅰand Ⅲ collagen syntheses and degradation in hypertrophic scars. Methods(1)Various concentrations of Resveratroal were added into the medium and co-incubated with FB for different time period.MTT assay was employed to detect proliferation of fibroblast.(2)With Resveratraol was performed on hypertrophic scars.On the 3rd day and 7th day,type Ⅰ and Ⅲ procollagen mRNA expressions were studied by means of immunohistochemistry and molecular biology. Results(1)The data of the present study demonstrated that Resveratraol in various concentrations inhibite proliferation of fibroblast.(2)On the 3rd day after with Resveratraol.Both type Ⅰand Ⅲ procollagen mRNA expressions were significantly inhibited and on the 7th day both procollagen mRNA expressions decreased further.With Resveratraol may inhibit type Ⅰprocollagen mRNA expression more than type Ⅲ. Conclusions Resveratraol may inhibit viability of fibroblasts and type Ⅰand Ⅲ procollagen mRNA expression.更多还原