【摘要】 目的探讨斑点金免疫渗滤法(DIGFA)、酶联免疫吸附试验(ELISA)与间接红细胞凝集试验(IHA)在日本血吸虫病流行区平行检测居民血清抗体的应用价值。方法采用DIGFA、ELISA和IHA平行检测粪检血吸虫虫卵阳性血清、健康人群及其它3种寄生虫感染人群血清、血吸虫病流行区居民血清,并对检测结果进行统计分析。结果DIGFA、ELISA和IHA检测100例粪检血吸虫虫卵阳性血清的敏感性均为96.0%;检测188例健康人群及其它3种寄生虫感染人群血清的特异性分别为98.9%、96.8%和97.3%;平行检测血吸虫病流行区居民血清抗体的阳性率分别为9.35%(102/1091)、11.54%(125/1083)和11.00%(120/1091),检测结果在统计学上无显著性差异(P>0.05)。结论DIGFA、ELISA和IHA敏感性高、特异性强,交叉反应少,平行检测血吸虫病流行区居民血清抗体的阳性检出率较为符合。更多还原

【Abstract】 To explore application values of detection serum antibodies to Schistosoma japonicum of population in epidemic area of schistosomiasis by means of DIGFA, ELISA and IHA, these method of tests were used in parallel to detect the presence of anti-S.japonicum antibodies in the fecal eggs positive patients with schistosomiasis and patients with paragonimiasis, ancylostomiasis and ascariasis as well as the healthy individuals in non-epidemic area and the inhabitants of the epidemic area. It was found that the sensitivities of these three methods for the detection of Anti-S.japonicum antibodies in 100 cases of egg-positive patients were all to be 96.0% (96/100),and the specificities of them as demonstrated in 188 cases of healthy individuals and other three parasitic infections were 98.9%(186/188), 96.8% (182/188) and 97.3% (183/188) respectively. The positive rates of antibodies from inhabitants in epidemic area were 9.35% (102/1091), 11.54% (125/1083) and 11.00% (120/1091) respectively. No obvious difference was found among the results of these three methods. It is concluded that DIGFA,ELISA and IHA all show high sensitivity and specificity with a low rate of cross-reaction, and are suitable for the detection of anti-S.japonicum antibodies in the epidemic area.更多还原