【摘要】 目的检测急性感染者血清中的登革病毒,并判定其型别。方法从血清中提取病毒RNA,使用通用引物和型特异性引物,用逆转录-套式PCR方法扩增登革病毒特异性核酸片段,电泳后观察判定型别。同时还将扩增产物进行测序分析。结果用通用引物扩增5份标本,均出现511bp扩增带,在型特异性引物的扩增下均出现482bp的扩增带,初步判断为DVⅠ病毒。经测序分析进一步确定为DVⅠ病毒。结论运用此方法证实2004年福建省登革热流行系DVⅠ病毒引起。更多还原

【Abstract】 The RNA extracted from sera of patients with acute infection was used to synthesize cDNA in RT-PCR with a pair of universal primers, and the serotype of virus products yielded in second round PCR amplification with a pair of type specific primers was analyzed . The sequence of the amplified products were analyzed at the same time. By using of these methods the amplified products with nucleotide length of 511 bp and 482 bp were obtained, and the Dengue type 1 virus was identified preliminarily by RT-PCR. In addition, the product with universal primers was further confirmed by sequencing to be the Dengue type 1 virus. It is evident that the prevalence of Dengue fever on 2004 in Fujian is caused by the Dengue type 1 virus.更多还原