【摘要】 目的:探讨小鼠接种结核菌H37Ra株后,巨噬细胞表达TNF -α的活性情况。方法:H37Ra皮内、腹腔途径免疫小鼠后第30、6 0天,采用生物学活性方法检测小鼠腹腔巨噬细胞表达的TNF -α活性。结果:小鼠接种H37Ra后,30天时TNF-α活性分别为2 2 .0 6U/ml(腹腔)和2 2 .6 3U/ml(皮内) ;6 0天时TNF -α的活性分别为36 .76U/ml(腹腔)和33.78U/ml(皮内) ,明显高于未免疫组(P <0 .0 1) ;与BCG皮内免疫组比较,30天时无显著性差异(P >0 .0 5 ) ,6 0天时有明显差异(P <0 .0 5 )。结论:H37Ra免疫小鼠后能诱导机体产生TNF -α。更多还原

【Abstract】 Objective:To explore the activity of tumor necrosis factor-α(TNF-α)from celiac macrophage after immunization of H37Ra in mice.Methods:The activity of TNF-α from celiac macrophage was measured by biological method at 30 th day and 60 th day after subcutaneous immunization and celiac immunization of H37Ra in mice.Results:After immunization of H37Ra in mice,the activity of TNF-α by celiac immunized route and subcutaneous immunized route was 22.06U/ml and 22.63U/ml respectively at 30 th day;and it was 36.76U/ml and 33.78U/ml respectively at 60 th day.The activity of TNF-α immunized with H37Ra at 30 th day and 60 th day was much higher than that of non-immunization group( P <0.01).There was no statistically significant difference between H37Ra immunization group and BCG immunization group at 30 th day( P >0.05),however,the activity of TNF-α in H37Ra immunization group was much higher than that in BCG immunization group at 60 th day( P <0.05).Conclusion:H37Ra can induce TNF-α.更多还原