【摘要】 目的　探讨一种理想的大鼠急性肝衰竭 (AHF)模型建立方法。方法　SD大鼠 3 6只随机分为 3组 :(1)改良手术诱导组 ,切除约 95 %肝脏组织 ,术中经中叶肝静脉注入 5 %葡萄糖氯化钠溶液 10ml/kg体重 ;(2 )传统手术诱导组 ,术中不行中叶肝静脉穿刺注射 5 %葡萄糖氯化钠溶液 ,其余手术方法同改良手术组 ;(3 )药物诱导组 ,给予D -氨基半乳糖 (D gal) 1.2g/kg腹腔注射。观察手术死亡率、建模后 2 4h大鼠存活率、血谷丙转氨酶 (ALT)、血氨 (NH3)、总胆红素 (TB)和血糖 (BG )。结果　传统手术诱导组手术死亡率高于改良手术组死亡率 (3 3 .3 %∶0 % ) ,改良手术诱导组、传统手术诱导组、药物诱导组大鼠建模成功后 2 4h存活率分别为 0 % ,0 % ,2 5 % ,改良手术诱导组ALT和NH3水平显著高于药物诱导组 (P <0 .0 5 ) ,TB和BG水平低于药物诱导组 ,但差异无显著性 (P >0 .0 5 )。结论　通过改进的 95 %肝切除术可建立较理想的大鼠AHF模型 ,术中经中叶肝静脉注入 5 %葡萄糖氯化钠溶液可减少手术死亡率。更多还原

【Abstract】 Objective To explore an ideal method of establishing a rat acute hepatic failure (AHF) model. Methods 36 Sprague Dawley rats were randomly divided into three groups. In modified operation induction group, rat AHF model was established by modified 95% hepatectomy. In the operation, 10ml 5% glucose saline solution per kilogram of body weight was injected into the median lobe hepatic vein of the rat. In traditional operation induction group, 95% liver was resected, but no glucose saline solution was injected. In drug induction group, AHF model was established by injecting 1.2g D gal per kilogram of body weight intraperitoneally . Operative mortality and 24h survival rate after establishment of models were observed. ALT, total bilirubin (TB), NH 3 and blood sugar (BG) were monitored. Results The operation mortality in traditional operation induction group was higher than that in modified operation induction group (33.3% vs 0%). Survival rate was 0%, 0%and 25% in modified operation induction group, traditional operation induction group and D gal group 24h later, respectively. ALT and NH 3 in the modified operation induction group were significantly higher than those of D gal group (P<0.05). TBIL and BG levels in the modified operation group were lower than those in the D gal group, but the difference was not significant (P> 0.05 ). Conclusions The AHF model established by modified 95% hepatectomy is an ideal one. Injection of 5% glucose saline solution through median lobe hepatic vein at operation can reduce operative mortality.更多还原