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不同蔬菜连作对土壤细菌DNA分子水平多态性影响的研究

Effct of Continuous Cropping of Different Vegetables on DNA Polymorphorism of Soil Bacterial

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【作者】 雷娟利周艳虹丁桔王礼喻景权

【Author】 LEI Juan-li1,2, ZHOU Yan-hong1, DING Ju1, WANG Li1, YU Jing-quan1 (1Department of Horticulture, Zhejiang University, Hangzhou 310029; 2Vegetable Institute, Zhejiang Agricultural Sciences, Hangzhou 310021)

【机构】 浙江大学园艺系浙江大学园艺系 杭州310029 浙江省农业科学院蔬菜研究所杭州310021杭州310029杭州310029

【摘要】 采用从土壤中直接提取微生物总DNA,并用细菌16SrDNA特异性引物进行PCR扩增、变性梯度凝胶电泳(DGGE)、克隆和测序等一系列分子生物学手段,研究了不同蔬菜连作对土壤中细菌群落结构的影响。结果表明,采自同一地区的土壤样品,其DGGE图谱的相似性很高;同时蔬菜连作对土壤中细菌的群落组成产生影响,这种影响同蔬菜作物种类和连作年限有关。研究结果还表明,所取土样中的细菌大多数为Proteobacteria类细菌,另外Acidobacteria、Sphingobacteria和Actinobacteria类细菌只占少量比例。

【Abstract】 Soil bacterial communities were examined by culture-independent means, namely, 16S rDNA-nested PCR-DGGE profiling. Crude DNA was extracted from soil grown with different vegetables for different years, amplified with bacterial specific primers of 16S rDNA by PCR, and separated by denaturing gradient gel electrophoresis (DGGE). Subsequently, bands on DGGE gels were cloned, sequenced and the resultant information was used to infer the diversity of bacteria in different soils. The results showed that soil samples collected from same area had the high identity in DGGE bands patterns. Bacterial community structure was significantly influenced by continuous cropping system. The effects were closely related to the kind of vegetables and the history of continuous cropping. The sequence results showed that most of the bacteria belong to Proteobacteria; Acidobacteria, Sphingobacteria and Actinobacteria also appeared in the continuously cropped soils.

【关键词】 蔬菜连作土壤细菌16SrDNAPCR-DGGE
【Key words】 Vegetable speciesSoilBacterium16S rDNAPCR-DGGE
【基金】 国家自然科学基金重点项目(3023960)
  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2005年10期
  • 【分类号】S63
  • 【被引频次】81
  • 【下载频次】751
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