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应用Charleston×东农594重组自交系群体构建SSR大豆遗传图谱

Construction and Analysis of Soybean Genetic Map Using Recombinant Inbred Line of Charleston × Dongnong 594

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【作者】 陈庆山张忠臣刘春燕王伟权李文滨

【Author】 CHEN Qing-shan1, ZHANG Zhong-chen1, LIU Chun-yan1, WANG Wei-quan2, LI Wen-bin1 (1Soybean Research Institute, Northeast Agricultural University, Harbin 150030; 2Sun Yat-sen University, Guangzhou 510275)

【机构】 东北农业大学大豆研究所中山大学东北农业大学大豆研究所 哈尔滨150030哈尔滨150030广州510275哈尔滨150030

【摘要】 以美国半矮杆大豆品种Charleston为母本,东北农业大学高蛋白大豆品系东农594为父本及其F2:10代重组自交系的154个株系为试验材料,利用164个在亲本之间表现多态的SSR引物对群体进行了分析,构建了一张大豆遗传图谱。该大豆遗传图谱总长度1913.5cM,标记间平均距离为11.89cM。每个连锁群长度变动在0.4~309.5cM之间,连锁群上的标记数在2~28个之间。各连锁群上的SSR标记并不是均匀分布的,其中A1、C2、D1a三个连锁群存在标记密集区。与国内外已构建完成的5张大豆遗传图谱比较表明,该图谱与国外的大豆公共遗传图谱对应性较好。

【Abstract】 F2:10 RIL population with 154 lines, crossed by Charleston as female parent and Dongnong 594 as male parent were used in this experiment. One hundred and sixty-four SSR primers were screened with the two parents and amplified on the 154 lines. A new soybean molecular genetic map, named NEAUSRI-GMS, was constructed by Mapmaker. The total length of the soybean genetic map is 1913.5 cM, and the average distance among markers is 11.89 cM. The length of linkage group varied from 0.4 cM to 309.5 cM, and the markers on the linkage group varied from 2 to 28. The distribution of SSR markers on every linkage group is not even. High density region of markers existed on linkage group A1, C2, and D1a. Compared with 5 soybean genetic maps constructed at home and abroad, NEAUSRI-GMS has high homologous with the public genetic map abroad.

【关键词】 大豆遗传图谱SSR构建
【Key words】 SoybeanGenetic mapSSRConstruction
【基金】 国家“863”计划(2001AA211041);哈尔滨市青年科学研究基金(2002AFQXJ043);国家科技攻关项目(东北区2004BA907A26)
  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2005年07期
  • 【分类号】S565.1
  • 【被引频次】111
  • 【下载频次】667
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