【摘要】 小麦多酚氧化酶(PPO)活性是引起面条和面团储存期间颜色褐变的主要因素。发掘可应用于籽粒PPO活性辅助选择的分子标记,将有助于小麦面粉颜色性状的遗传改良。本研究选用来自全国不同麦区的203份冬小麦品种,验证SSR(simplesequencerepeat)引物Xgwm312的PCR扩增片段大小与籽粒PPO活性的关系。结果表明,198bp扩增片段的有无同籽粒PPO活性大小密切相关,该片段的出现通常意味着籽粒PPO活性较高。Xgwm312可应用于籽粒PPO活性的分子标记辅助育种。更多还原

【Abstract】 Polyphenol oxidase (PPO) is highly related to undesirable brown discoloration of wheat-based end products such as noodles. It is important to identify molecular markers associated with PPO activities for marker-assisted selection (MAS) for wheat quality improvement. A total of 203 Chinese winter wheat cultivars and lines were analyzed to investigate the correlation of PPO activities with a SSR(simple sequence repeat)marker Xgwm312. The results indicated that a fragment with 198 bp is apparently correlated with high PPO activities in wheat grain. The marker Xgwm312 can be used for MAS for PPO activities.更多还原