【摘要】 对灰葡萄孢BC4菌株进行紫外线诱变得到了15个诱变菌株,经测定筛选出BC4-1、BC4-2和BC4-15等3个诱变菌株中以BC4-1菌株效果最好。试验发现,用于毒素产生的PD培养基的pH值对产毒能力影响很大,以pH4.0最优。用三氯甲烷、石油醚、乙酸乙酯萃取培养滤液制备BC-粗毒素,生物测定结果表明,以乙酸乙酯萃取所得粗毒素对杂草种子萌发及生长抑制作用最强。通过柱层析法和HPLC法分离和纯化除草活性组分,得到1个在271nm有最大UV吸收的化合物,该组分在100mg·L-1浓度下对马唐的生长具有强烈的抑制作用,在50mg·L-1浓度下可完全抑制马唐和反枝苋种子的萌发。更多还原

【Abstract】 Fifteen mutant isolates were obtained by ultraviolet ray mutation from parent strain Botrytis cinerea BC-4 and 3 mutant isolates, i.e. BC4-1, BC4-2 and BC4-15, showed strong herbicidal activity, among them, BC4-1 was the best for inhibiting the germination and growth of Digitaria sanguinalis L. and Amaranthus retroflexus L. The results also showed that different pH of PD media had different influences on herbicidal activity and pH 4.0 was the best among the pH values tested. The crude toxin could be extracted using chloroform, petroleum ether and ethyl acetate, and the ethyl acetate extracts had a strongest inhibitory activity on the germination and growth of D. sanguinalis L .and A. retroflexus L. One component with an absorbent peak at 271 nm was separated and obtained from the crude toxin by HPLC. The component, at concentration of 100 mg·L-1, could strongly inhibit the growth of D. sanguinalis L. and could completely inhibit seed germination of D. sanguinalis L. and A. retroflexus L.at concentration of 50 mg·L-1.更多还原