【摘要】 枯、黄萎病是世界棉花生产中的两大重要病害。传统育种缺乏抗源,几丁质酶和β-1,3-葡聚糖酶是植物防御体系中的两种防卫因子,两者之间存在协同增效作用。据此构建了4个单价和2个双价基因(分别定位于细胞内或细胞外)的植物表达载体,通过花粉管通道法转化棉花,经PCR和Southern杂交检测以及1996～2000年温室及病圃多代筛选鉴定,已培育出对枯、黄萎病抗性提高的转基因棉花株系。将抗病基因导入国产抗虫棉品种GK19中,还获得了兼抗病、虫的转基因优系。更多还原

【Abstract】 Fusarium and Verticillium wilt are two important diseases in cotton in the world. Traditional breeding method does not have resistance germplasm. Chitinase and β-1,3-glucanase are two factors with synogestic effects involved in the plant defense system. Based on this fact, a series of plant expression vectors either harboring chitinase gene or β-1,3-glucanase gene or both were constructed. The two genes are expected to be expressed and localized in vacuole or in extra-cellular space. The transformation of cotton was conducted by pollen-tube pathway method. Molecular analysis of transgenic cotton plants by polymerase chain reaction and Southern hybridization, and successive repeated selection of transgenic cotton lines in greenhouse and fungi-infected nurseries during the period of 1996-2000 demonstrated that the transgenic cotton lines with Fusarium wilt and Verticillium wilt resistance were obtained. Meanwhile, the above anti-fungal genes have been integrated into insect-resistant cotton variety GK19 which resulted in the development of transgenic lines resistant to both cotton boll worm as well as Fusarium and Verticillium wilt.更多还原