【摘要】 用RAPD标记方法对来自中国、日本、韩国、不丹、尼泊尔、越南6国163份小豆种质资源DNA的遗传多样性进行检测。从27个引物共检测到285条带,有261条具多态性(占92.98%),其中野生型多态性带谱占85.82%,栽培型次之为83.52%,半野生型为80.46%,三类型小豆都有其自身特征带;遗传离散度大小顺序是:野生型>半野生型>栽培型,其遗传分化系数大小顺序为:野生型<半野生型<栽培型;从分化系数差异看,半野生型小豆更接近野生型小豆;从相似系数平均值来看,半野生型小豆材料之间亲缘关系较近,而处在进化两极的野生型材料之间、栽培型材料之间亲缘关系都较远;来自我国西南以及日本南部两地区的小豆材料遗传离散度较大,遗传分化系数、相似系数平均值较低。聚类分析结果表明,163个小豆材料以遗传相似系数0.32为截值,可分为8大类,归类有较明显的地理相关性及遗传类型的趋同性。更多还原

【Abstract】 RAPD variation was assessed in 163 accessions of adzuki bean from China, Japan, Korea, Bhutan, Nepal and Vietnam. 285 bands, including 261 polymorphic bands, were detected by 27 RAPD primers. Among those bands observed in wild, weedy and cultivated adzuki bean, the rates of polymorphism were 85.82%, 80.46% and 83.52%, respectively. There were species-specific bands in the three races. The order of genetic dispersion was: wild adzuki bean>weedy adzuki bean>cultivated adzuki bean, and the order of Gst was: wild adzuki bean<weedy adzuki bean<cultivated adzuki bean. The weedy adzuki bean has close genetic relationship with wild adzuki bean assessed by the difference of genetic dispersion. From the average of genetic similarity coefficient, a relative close genetic relationship was observed in the weedy adzuki bean, whereas the wild and cultivated adzuki bean, as two pole of evolution, has high genetic variation. The material from Southwest China and South Japan has relatively high genetic dispersion, low Gst and average of genetic similarity coefficient. The cluster analysis showed that eight groups can be clustered when genetic similarity coefficient was given as 0.32. It appears that the cluster has obviously associated with the genetic races and different adzuki bean geographical regions.更多还原