【摘要】 采用自制多克隆抗体,考察了不同离子强度和不同甲醇含量对竞争反应的影响,并对反应体系进行了优化,最终建立了黄瓜中甲基对硫磷的残留检测ELISA方法。实验表明,甲基对硫磷对抗体竞争性结合的I50为0.13mg/L,方法检出限I20为6.7μg/L。除倍硫磷与杀螟硫磷表现了一定的交叉反应外,其它结构相类似的有机磷农药均无交叉反应。黄瓜样品经简单前与处理后分别采用ELISA和GC测定,用ELISA方法测得黄瓜中甲基对硫磷的添加回收率为83.10%￣93.46%,C.V为6.61%￣10.36%,最低检出限为0.003mg/kg,与GC测定数据基本相符,符合农药残留分析的要求。更多还原

【Abstract】 Using the self-made poly-clonal antibody, the affect of the ion-strength and the methanol concentration to the competitive reaction were investigated, and the reaction system was optimized. An indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of parathion-methyl in cucumber was developed. The results showed that the concentration of parathion-methyl that inhibits 50% of antibody-antigen binding(I50)was 0.13 mg/L, the detection limit I20 was 6.7 μg/L. Most analogues showed negligible cross-reactivity except for fenthion and fenitrothion. Cucumber samples were detected with ELISA and GC after simple pre-procession. The fortified recovery of ELISA were 83.10%~93.46%,CV were 6.61%~10.36%,the determination limit was 0.003mg/kg. The results of the ELISA were verified by GC method.更多还原