【摘要】 为了找到提取竹叶中叶绿素的最佳工艺条件,考察竹叶叶绿素的稳定性,通过单因素实验确定浸提溶剂种类原料颗粒度、料液比、浸提温度、浸提时间的合适范围,再利用正交试验法进行工艺优化.结果表明,用无水乙醇提取竹叶叶绿素的最佳工艺条件是:原料颗粒度10～20目、料液比1∶5、浸提温度70℃、浸提时间7h,竹叶叶绿素对紫外光、常见氧化剂(如H2O2)、Fe3+均具有较强的耐受性,在温度70℃以下以及近中性或偏碱性介质环境中性质基本稳定,遇Cu2+、Zn2+离子分别生成蓝绿色絮状沉淀和灰绿色颗粒状沉淀.更多还原

【Abstract】 <Abstrcat>The objective of the research is to obtain the optimal condition for the extraction of chlorphyl from bamboo leaves, and to evaluate its stability. The applied methods consist of finding the solvent and exploring the reasonable ranges of the granularity of material, the ratio of material to solvent, temperature and time based on single factor experiments, and then optimizing the extraction process by orthogonal tests. Results show that with absolute ethyl alcohol as the solvent, the optimal condition for the extraction was obtained as follows: the granularity of material 1.2～1.3 mm, the ratio of material to solvent 1∶5, temperature 70℃, time 7 hours. The chlorphyl from bamboo leaves showed strong resistibility to ultraviolet rays, common oxidants （e.g. H₂O₂） and Fe³⁺. When the temperature was lower than 70℃ or pH≥6, the properties of bamboo chlorphyl were almost unconverted. There would accordingly be bluish-green depositions and greenish-gray ones with the existence of Cu²⁺ and Zn²⁺. The result is very useful to the usage and further development of the chlorphyl from bamboo leaves.更多还原