【摘要】 目的探讨β胡萝卜素(β-Carton,β-C)与维生素E(VitaminE,VE)不同配比方案,对荷瘤小鼠放、化疗后血液学及肿瘤生长的影响,确定其减轻放、化疗毒副作用的合理剂量配比水平。方法采用有重复的正交实验设计,用L8(27)正交表研究β-C、VE不同水平的两两配比及放疗、化疗2种治疗方案的不同配比对荷瘤小鼠放、化疗后血液白细胞、血小板及瘤重的影响。24只小鼠随机分为8组,经口给予不同剂量配比的β-C、VE,20天后每只小鼠腋下接种S-180瘤液0.2ml,接种瘤液后第2天对小鼠分别进行化疗、放疗。放疗组为一次性全身γ-射线辐射,剂量为4Gy,化疗组给予环磷酰胺,剂量为180mg/kg·bw,隔日1次,共5次。治疗前一天及治疗后第3天进行白细胞计数及血小板计数,治疗后第10天解剖动物,检测动物的肿瘤重量,计算瘤体比。结果单因素β-C在抑制由于放、化疗引起的白细胞、血小板下降及抑制肿瘤生长方面作用明显(P<0.05);单因素VE可明显抑制荷瘤小鼠的肿瘤生长(P<0.05);β-C和VE交互作用可抑制荷瘤小鼠由于放、化疗引起血小板下降(P<0.05),并明显抑制荷瘤小鼠肿瘤生长(P<0.05)。结论β-C+VE在抑制荷瘤小鼠由于放、化疗引起血小板下降方面合理的剂量配比为β-C60mg/kg+VE50mg/kg;β-C+VE抑制荷瘤小鼠肿瘤生长的合理剂量配比为β-C30mg/kg+VE50mg/kg(以每公斤体重计)。更多还原

【Abstract】 Objective To get the best combination plan of β-Carotene and vitamin E. Different combination plans’ effects on haematology and the tumor weight of mice treated by chemotherapy or radiotherapy was explored. Methods Othogonal design (L8(27)) was used to observe the effects of the different combination plans of β-Carotene and vitamin E on haematology and the tumor weight of mouse after chemotherapy or radiotherapy. First, 24 mice were divided into 8 groups randomly and oral administered by different dose combination of β-Carotene and vitamin E. On the 21th days, the tumor cell (S-180) was implanted into the subaxillary tissue of mice. In the following day they were treated by chemotherapy (180 mg/kg·Bw/d×5 times), once every two days) or radiotherapy (4 Gy×1). The leucocytes and platelets were counted one day before treatment and at the 3 rd day after treatment. At the 10 th day after treatment, mice were sacrificed, and tumor was weighted. Results β-Carotene could restrain the decrease of the leucocyte and the growth of tumor (P<0.05). VE could inhibit the growth of tumor obviously too (P<0.05). The combination of β-C and VE restrained the decrease of platelets and inhibit the growth of tumor evidently(P<0.05). Conclusion The combination plan of β-Carotene (60 mg/kg·bw) and VE (50 mg/kg·bw) was better on restraining the decrease of platelets, and 30 mg/kg·bw of β-C with 50 mg/kg·bw of VE , was better on inhibiting the growth of tumor.更多还原