【摘要】 目的探讨老年急性局灶性脑缺血/再灌注后细胞凋亡相关基因Fas、FasL表达及caspase3活性的异同。方法采用线栓法制备急性局灶性脑缺血/再灌注模型,比较青年与老龄大鼠脑缺血3h及再灌流3、6、12、24、72h后Fas、FasL及caspase3活性变化情况。结果老龄大鼠脑缺血/再灌注后Fas、FasL的表达早于青年大鼠,表达强度高于青年大鼠。caspase3的激活与Fas、FasL表达的高峰期重叠或稍晚。老龄大鼠脑缺血/再灌注时caspase3的激活早于青年大鼠。结论老年急性局灶性脑缺血/再灌注后细胞凋亡相关基因表达特点为Fas、FasL的表达早,强度高,caspase3的激活早。更多还原

【Abstract】 Objective To explore the changes of Fas and FasL gene expression and caspase-3 activity after local cerebral ischemia/reperfusion (I/R) in the aged rats. Methods The acute local ischemia/reperfusion model was made with the intraluminal filament technique, Fas and FasL expression and caspase-3 activity of the young and the aged rats were compared at 3 hours of middle cerebral artery occlusion, followed by 3, 6, 12, 24, 72 h of reperfusion. Results The expression of Fas and Fas1 after I/R in the ischemic brain of the aged rats were earlier than that in the young rats. The intensity of expression was higher than that in young rats. Caspase-3 activation point was located or later in the fastigium of Fas and FasL. Caspase-3 activation in the aged rats after I/R was earlier than that in the young rats. Conclusions Expression of cellular apoptosis related gene in aged rats after I/R of the old rats could character as early expression of Fas and FasL with high intensity and early caspase-3 activation.更多还原