【摘要】 目的:采用改良传代法进行雪旺细胞培养,以获取大量纯净的雪旺细胞用于周围神经组织工程研究。方法:采用4-5 d SD乳鼠坐骨神经和臂丛神经,运用双酶消化法结合单酶消化法进行雪旺细胞的原代培养。5-7 d后,采用单酶快速消化离心法进行传代培养,同时纯化雪旺细胞。结果:SABC免疫组化染色鉴定和计数板计数,改良传代法使体外培养的雪旺细胞纯度达98％左有,两种方法之间有显著性差异,P<0.001。MTT检测雪旺细胞生存和增殖时间延长1-2周左右。结论:与传统的传代培养方法相比,改良法既省时又避免了外源性物质如Ara-C对雪旺细胞的毒性作用,同时有利于雪旺细胞活力和纯度的提高。更多还原

【Abstract】 Objective: To obtain highly purified and large amount of Scs by improved passage culture for nerve tissue engineering. Methods: Four to five-day-old SD rat’s sciatic nerve and brachial plexus were removed, double enzyme digest and single enzyme digest were adopted to primary culture of Scs respectively . Five-seven days later, Scs were purified by single enzyme digest and centrifuge quickly. Results: It was manifested that purification of Scs cultured by improved passage cultural method reached 98%(P<0.001). The period of SCs’ growth and proliferation checked by MTT extended 1-2 weeks compared with traditional method. Conclusions: Contrasting with the traditional method , this method saves times and avoid effecting on Scs from other substances (Ara-c,etc.), meanwhile activity and purification of Scs will be improved obviously.更多还原