【摘要】 目的探讨环氧化酶抑制剂尼美舒利对膀胱癌细胞系T24的凋亡诱导作用和对环氧化酶2(COX2)的表达抑制作用。方法以膀胱癌细胞系T24为研究对象,已知COX2高表达的胃癌细胞系SGC7901为阳性对照。分别经不同浓度尼美舒利作用25小时后,Westernblot检测COX2的表达;凋亡DNA琼脂凝胶电泳检测DNA凋亡梯度。ImageTool凝胶分析软件分析Westernblot电泳图像。结果DNA梯度随尼美舒利浓度的加大而加大,Westernblot结果显示COX2的表达随着尼美舒利的浓度加大而明显抑制,在浓度为100μmol/L时,无法检测到COX2的表达。ImageTool软件分析Westernblot灰度值差异有显著性(P<0.01)。结论尼美舒利有良好的诱导凋亡作用,其机制可能与COX2的表达抑制有关。更多还原

【Abstract】 Objective To study effects of nimesulide in inducing apoptosis and suppressing expression of cyclooxygenase-2(COX-2) in human bladder cancer cell line T24. Methods Human bladder cancer cell line T24 cells were studied and human gastric cancer cell line SGC-7901 was served as positive control cell line.Cells were cultured for 24 hours with nimesulide at different concentrations.Apoptosis was detected by DNA fragmentation electrophoresis, while COX-2 by Western blot. Image Tool software was used to analyze Western blot image. Results With increase of nimesulide concentrations, cell apoptosis increased.COX-2 expression could be obviously suppressed by COX-2 specific inhibitor nimesulide. The concentration of maximum suppressing effect was 100 μmol/L. Software analysis revealed that with the increase nimesulide concentrations, and the expression of COX-2 obviously decreased.Difference was significant(P< 0.01).Conclusion Nimesulide could effectively induced apoptosis in T24 cell line, which may be related to the expression inhibition of COX-2.更多还原