【摘要】 目的　探讨ER、PR、p53表达及DNA倍性在胆囊癌发生发展中的意义。方法　用免疫组化二步法及HPIAS 1000DAN图像分析系统检测27例胆囊癌组织中 ER、PR、p53 的表达及 DNA倍性。结果　①胆囊癌中ER、PR及 p53 的阳性表达率分别为77.78%(21/27)、74.07%(20/27)、66.67%(18/27). ②胆囊高分化腺癌中DNA倍体为高二倍体;中分化腺癌为高二倍体至亚四倍体;低分化腺癌为四倍体。③胆囊癌分化越差,浸润越深,其ER、PR、p53的阳性表达率越高(P<0.05),DNA含量越高,多倍体越明显(P<0.05),预后越差。结论　ER、PR、p53 阳性表达率及 DNA倍性改变与胆囊癌的分化程度和浸润深度有关。ER、PR过度表达可能会诱发 p53基因突变,两者在胆囊癌的发生发展中有密切的内在联系。更多还原

【Abstract】 Objective To investigate biological significance and relationship of ER、PR、p53 gene protein and DNA content in occurrence and development of gallbladder carcinoma. Methods The two steps of immunohistochemistry and HPIAS 1000 DNA image instrument were used to determine the expression of ER、PR、p53 and DNA content in 27 cases of gallbladder carcinoma. Results ① In 27 cases of gallbladder carcinoma , the positive expression rates of the ER, PR, p53 were 77.78 %、 74.07 % and 66.67 % , respectively. ② The DNA ploid in high, middle and low differentiation gallbladder carcinoma were over doubleploid, ranging from over doubleploid to sub quardriploid and sub quardriploid, respectively. ③ In gallbladder carcinoma, with the increasing of the invasive depth and the lowering degree of differentiation, the positive expression rate of ER, PR and p53 were rising up, so was the DNA contents. Conclusion The positive expression ER、PR、p53 and DNA changeful ploidesis is related to the level of differentiate extent and infiltrated depth of gallbladder carcinoma. Excessive expression of ER、PR will possibly induce the mutein of p53 gene. They have closely inside relationship in the occurrence and development of gallbladder carcinoma .更多还原