【摘要】 通过对高能量药物筛选基本过程的分析 ,探索了靶分子固定化可能对筛选质量构成的影响 .从分子相互作用看 ,实际的过程远较理想的情况复杂 .分子间存在的静电力、疏水作用力及氢键力能对固定化的机理构成较大影响 ,甚至可能诱发多层吸附的固定化模式 .基于固定化过程中可能存在的靶分子与靶分子、靶分子与微球、体相与表面相之间的相互作用 ,构筑了相应的数学物理模型 ,为实际的筛选工作提供了可参考的理论依据 .更多还原

【Abstract】 Based on the analysis of the basic process involved in high-throughpu t screening (HTS), the present article discusses the possible effect of target i mmobilization on the screening quality. Judged from intermolecular interactions , the actual process in HTS is more complicated than that in ideal situation. Wi th more or less interactions electrostatic, hydrophobic or hydrogen-bo nding ca n be involved in this process. With the induction derived from these interaction s, the immobilization mode of target may be in monolayer or a more c omplicated multi-layer form. In addition, this article presents the mathe m atic expressions related to the corresponding interaction forms, including targe t and target, target and microsphere, and bulk phase and surface phase as well. T hese expressions are expected to show a certain degree of usability in the futur e work.更多还原