【摘要】 目的观察β- 淀粉样肽(Aβ)25 35致痴呆小鼠模型脑单胺氧化酶B(MAO- B)、白细胞介素6(IL- 6)的改变及中药脑复聪的干预作用。方法脑室内注射Aβ25 35造成痴呆模型小鼠,用Morris 水迷宫测空间学习记忆功能;用比色法测MAO -B活性;免疫组化检测IL- 6。结果模型小鼠的Morris 水迷宫潜伏期(81. 3±13. 4) s,比对照组(34. 2±10 .9) s延长(P<0 .05);大脑皮层和海马的MAO B活性(120. 12±10 .15)、(83. 60±5 .29)均较对照组(93. 09±10 .54)、(50. 39±9 .16)升高(P<0 .05~0 .01),海马齿状回有大量IL- 6免疫荧光阳性细胞。经脑复聪治疗后,水迷宫潜伏期(43 7±12 7) s缩短(P<0 .05);海马的MAO B活性(47. 11±6. 57)回降(P<0 .01);IL- 6免疫阳性神经细胞减少。结论抑制海马MAO- B的过度激活,减少自由基的产生,减少氧化应激反应,抑制小胶质细胞产生炎性因子IL- 6,缓解Aβ的损伤作用,阻断AD发病的重要环节,是中药脑复聪治疗AD的重要机理之一。更多还原

【Abstract】 ObjectiveTo observe the changes of monoamine oxidase-B (MAO-B), interleukin-6(IL-6) in the cortex and hippocampus of Alzheimer’s disease model mice and the effect of traditional Chinese medicine Naofucong.MethodsAlzheimer’s disease model was induced in mice by β-amyloid(Aβ) 25-35icv. Space learning and memorial ability was tested in Morris water maze. The activity of MAO-B was measured by colorimetric method. IL-6 was observed with the immnuohistochemical stain.ResultsMice in the model group presented longer latent periods of Morris water maze(81.3±13.4) s, higher activities of MAO-B in brain cortex and hippocampus (120.12±10.15,83.60±5.29) compare with that of the control group, which was (34.2±10.9) s,(93.09±10.54) and (50.39±9.16)(P<0.05～0.01).There were many IL-6 positive cell in dentate gyrus of hippocampus of the model group. After administration with Naofucong grain, latent periods (43.7±12.7) s and activities of MAO-B (47.11 ± 6.57)in hippocampus were recovered(P<0.05～0.01), and the IL-6 positive cell in dentate gyrus decreased.ConclusionNaofucong grain can antagonize the Aβ 25-35toxicity by decreasing the overactivition of MAO-B and the excretion of inflammatory medium by microglia, as well as improve the memory function.更多还原