【摘要】 目的:探讨大鼠脊髓损伤后应用复方丹参对细胞凋亡及诱导型一氧化氮合酶(iNOS)表达的影响。方法:成年大鼠随机分为正常组、脊髓损伤后应用复方丹参组(组)和应用生理盐水对照组(组),损伤后不同AB时间点(8h、1d、3d、7d、14d、28d)处死大鼠,用HE染色观察损伤脊髓组织病理变化,原位末端标记法(TUNEL法)标记凋亡细胞,用免疫组化染色检测iNOS阳性细胞。结果:HE染色镜检发现脊髓组织病理学改变A组明显轻于B组。A、B两组均发现凋亡细胞及iNOS表达,神经细胞凋亡指数及iNOS表达均为B组>A组(P<0.01)。结论:复方丹参能抑制大鼠脊髓损伤后细胞凋亡及iNOS表达。更多还原

【Abstract】 Objective:To investigate the effect of salvia miltiorrhiza on apoptosis and expression of iNOS after spinal cord injury(SCI) in rats.Method:Adult SD rats were divided at random into three groups:with Allen′s weight drop to cause spinal cord injury and use salvia miltiorrhiza group(group A)? use normal saline group(group B) and normal group.After operation,the rats were sacrificed at 8 hours and 1?3?7?14?28 days after surgery,then the tissue sections were stained with hematoxylin and eosin,immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method,and the injured spinal cord tissue was observed.Result:The histopathology changes in spinal cord tissue observed with HE staining microscopic examination in group B was worse than that in group A.Expression of apoptotic cells and iNOS were observed in the two groups,and the apoptotic index and expression of iNOS on group B is higher than that in group A(P<0.01).Conclusion:Salvia miltiorrhiza could inhibit apoptosis and expression of iNOS after spinal cord injury in rats.更多还原