【摘要】 目的了解超排卵周期人血清及卵泡液中胰岛素样生长因子II(IGF-II)水平,探讨IGF-II在生殖内分泌中的作用。方法采用放射免疫法(RIA)测定45例超排卵周期取卵日人血清及卵泡液中IGF-II、雌二醇(E2)、孕酮(P)、睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)水平,分析IGF-II与卵泡发育、生殖激素及为超排卵而使用r-FSH、人绝经期促性腺激素(hMG)的剂量之间的相关性及意义。结果卵泡液IGF-II(0.60±0.69)μgL,低于血清IGF-II(1.09±1.21)μgL(z=1.975,P<0.05);卵泡液中IGF-II与E2、P水平呈正相关(r=0.620、0.509,均P<0.01);与TE2比值呈负相关(r=0.514,P<0.01),与FSH、LH水平及LHFSH比值无相关性(r=0.033、0.044、0.062,均P>0.05);与直径14mm以上的卵泡数及获卵数呈正相关(r=0.393、0.324,均P<0.05)。血清IGF-II与E2、P、T、FSH、LH水平及TE2均无相关性(均P>0.05),与直径14mm以上的卵泡数及获卵数亦无相关性(r=0.099、0.185,均P>0.05)。血清及卵泡液IGF-II水平与超排卵而使用的r-FSH、hMG剂量均无显著相关性(均P>0.05)。结论IGF-II在卵巢内调节卵巢功能中起了重要作用,可能具有促进卵泡发育、形成优势卵泡的作用,并可能促进卵泡E2、P的分泌。更多还原

【Abstract】 Objective To measure the levels of insulin-like growth factor II IGF-II in serum and follicular fluid FF of patients during superovulation stimulation period and to investigate the relationships of IGF-II levels with follicular development and reproductive hormones in order to discuss the significance of IGF-II in reproductive endocrine Methods The serum and follicular fluid samples were collected from 45 patients who received in-vitro fertilization IVF and embryo transfer ET at the time of transvaginal oocyte retrieval after ovulation induction. The levels of IGF-II estradiol E2,progesterone P testosterone T follicle-stimulating hormone FSH and luteinizing hormone LH in serum and follicular fluid were measured by radioimmunoassay RIA. The correlations between the IGF-II levels and above hormone levels the amounts of r-FSH?hMG used for superovulation and follicular development were analyzed with SPSS 10.0 software. Results The concentration of IGF- II in FF(0.60±0.69)μg L was lower than that in the serum (1.09±1.21)μg LP<0.01. It was found that the IGF-II level in FF was positively correlated with E2 or P levels P≤0.001 and the number of follicles ≥14 mm diameter or retrievable ova P<0.05 but negatively correlated with T E2 in FFP=0.001 and no significant correlation with FSH?LH levels and LH/FSH. On the other hand the IGF-II levels in serum showed no significant correlation with these hormone levels and the number of follicles ≥14 mm diameter or retrievable ova P>0.05. The IGF-II levels in the serum and FF showed no significant correlation with the amounts of r-FSH and hMG used for superovulation(P>0.05). Conclusion IGF-II may promote ovarian follicular development and dominant follicle selection probably and stimulate E2 and P production by follicles. As an important biological regulator in human ovary IGF-II may play roles in intraovary environment. The levels of IGF-II in human body could not show significant statistical correlation with gonadotropins.更多还原