【摘要】 目的:观察活体情况下微囊藻毒素LR暴露对大鼠肝脏P53、Bcl-2和Bax蛋白表达的影响。方法:SD大鼠灌胃染毒,运用Westernblot检测大鼠肝脏P53、Bcl-2和Bax蛋白表达情况。结果:与对照组相比,除了0.1μg/kgLR暴露实验组外,其余各实验组P53和Bax表达都明显升高(P<0.05),而且随着微囊藻毒素LR暴露浓度的升高呈升高趋势;Bcl-2表达没有明显的变化。结论:在活体情况下P53和Bax在微囊藻毒素LR诱导的细胞凋亡中可能起重要作用,而Bcl-2似乎不参与这一过程。更多还原

【Abstract】 <Abstrcat>Objective: To examine the expression of P53,Bcl-2 and Bax proteins in rat liver after exposed to microcystin LR. Methods: SD rats received microsystin LR by gastric perfusion.The expression of P53,Bax and Bcl-2 in liver was detected by Western blot. Results: The expression of P53 and Bax in each treatment group increased significantly compared with that in control group(P<0.05),with the exception of0.1μg/kg LR exposure group.Moreover,with exposure levels increasing,the expression of P53 andBax increased gradually; while no changes of the expression of Bcl-2 were observed. Conclusion: P53 and Bax may play important roles in microcystin LR-induced apoptosis,but Bcl-2 seems not be involved in this process.更多还原