【摘要】 目的:研究环氧化酶2(cyclooxygenase2,COX2)抑制剂celecoxib对人骨肉瘤细胞MG63抑制增殖及诱导凋亡的作用。方法:MTT比色观察celecoxib的细胞毒性作用及其浓度依赖性;Hoechst33258荧光染色、透射电子显微镜和TUNEL观察细胞凋亡的形态学变化;流式细胞仪检测细胞凋亡率及其时间依赖性。结果:celecoxib分别以10、20、40、80μmol/L作用后,细胞生长受到不同程度抑制;荧光显微镜、电镜和TUNEL观察到细胞胞浆浓缩、核凝聚、核碎裂和凋亡小体形成。流式细胞仪显示celecoxib40μmol/L作用24、48、72h后,细胞凋亡率分别与对照组比较有非常显著性差异(P<0.01)。结论:celecoxib能抑制人MG63细胞增殖,诱导细胞凋亡。更多还原

【Abstract】 Objective:To study and detect the effect of celecoxib on anti-proliferation and inducing apoptosis of human osteosarcoma cell MG-63 line in vitro.Method:Growth suppression was detected by MTT method.Special changes of apoptosis in morphology were observed by fluorescence microscopy,transmission electron microscopy (TEM) and TDT-mediated dUTP-biotin nick end-labeling (TUNEL) assay.The apoptosis rates were quantified by flow cytometry (FCM).Result:The growth inhibition rate of MG-63 cells treated with 10,20,40,80μmol/l celecoxib were 5.16%,16.53%,48.31%,87.76% respectively(P<0.05,P<0.01).Through TEM and TUNEL assay,the special morphological changes were observed,such as nuclear chromatin condensation,fragmentation,cell shrinkage,and the formation of apoptotic bodies.At different time of 24,48 and 72 hours,the apoptotic rates of MG-63 cells treated with celecoxib 40μmol/l were respectively,significantly higher than that of the control group (P<0.01).Conclusion:Celecoxib could inhibit the proliferation of human osteosarcoma cell MG-63 line and induce the cell apoptosis.It is a very interesting subject to further investigate the mechanism.更多还原