【摘要】 根据prp1-1启动子序列设计并合成了一对引物,通过PCR扩增的方法从马铃薯基因组中扩增到了病原诱导的prp1-1启动子,并将其克隆到PGEMT-Easy载体上,然后进一步克隆到植物表达载体pBI101.2上,构建了病原诱导型基因表达载体pBP.更多还原

【Abstract】 According to the reported sequences of inducible expression promoter prp1-1,a pair of primers were designed and synthesised.After the fragment of prp1-1 promoter was amplified by the method of PCR from the potato,it was inserted into PGEMT-Easy vector.Thereafter,the fragment was cloned into pBI101.2 expression vector,and a new kind of pathogen-inducible expression vector pBP was constructed.更多还原