【摘要】 目的探讨东方田鼠血清不同组分对日本血吸虫童虫的杀伤力,以期发现东方田鼠血清中参与其天然抗日本血吸虫的相关蛋白质。方法以离子交换柱层析及分子筛柱层析技术对东方田鼠血清蛋白质进行分离,将所得血清不同组分加入体外培养的2日龄日本血吸虫童虫中(100±20条/孔),计算童虫死亡率以判断血清不同组分对童虫的杀伤力。结果从东方田鼠血清分离出58个蛋白组分,其中6个组分具有明显的杀伤日本血吸虫童虫的活性,童虫死亡率均在37%以上,特别是组分18.1的童虫死亡率达87.5%,而阴性对照组童虫死亡率为25.0%(P<0.01)。结论东方田鼠血清中某些蛋白质参与其抗日本血吸虫的过程。更多还原

【Abstract】 Objective To explore the killing effect of different fractional proteins from Microtus fortis(Mf) serum to S.japonicum juveniles,and to find possible association of the proteins with the natural resistance to schistosome infection.Methods The proteins in Mf serum were separated by means of ion,exchange column chromatography and molecular sieve column chromatography.After desalted by dialysis and lyophilized,the proteins were dissolved in DMEM medium which contained 300 U/ml penicillin and 300 μg/ml streptomycin,and the two,day old schistosomula were added in for in vitro cultivation(100±20/well).The killing activity of the fractional proteins to the juvenile worms was defined by mortality rate.Results 58 fractional proteins were separated from Mf serum,in which six proteins were confirmed to have a significant killing activity to schistosomula.The mortality of schistosomula all reached 37% and above,and the highest mortality(87.5%) was observed in the fraction 18.1,while the negative control was 25.0%(P<0.01).Conclusion Some fractional proteins in Microtus fortis serum show an effect in the natural resistance to schistosome infection.更多还原