【摘要】 将猪繁殖与呼吸综合征病毒(PRRSV)的核衣壳蛋白(N蛋白)基因克隆到原核表达载体pET 28α(+)中,得到重组质粒pET PRRSV/N,并转化入受体菌BL21中。转化子经IPTG诱导表达4h后,表达蛋白量达到高峰。经15%SDS PAGE电泳检测和经Westernblotting分析,表达蛋白大小约为17kD,符合预期结果,并能与PRRSV阳性血清发生特异性反应,而不与阴性血清反应。这说明表达蛋白具有高度免疫反应特异性。更多还原

【Abstract】 <Abstrcat>The nucleocapsid protein(N) gene of porcine reproductive and respiratory syndrome virus(PRRSV) was cloned into the multiple cloning site of pET-28α(+) vector.The recombinant of pET-PRRSV/N was cultivated and induced with IPTG.The amount of expressed protein reached a high peak 4 hours after the induction. The examination analysis results of the protein by using SDS-PAGE and Western blotting showed that the protein was 17 kD in size and specifically reacted with PRRS-positive sera from pigs,not negative sera,indicating the recombinant protein had high specificity of immune reaction and can be used as antigen of diagnostic assay for PRRS in practice.更多还原