【摘要】 目的观察苦参碱的体内抗肿瘤活性,初步探讨其体内抑瘤作用机制。方法采用小鼠H22肝癌细胞实体瘤模型进行体内抑瘤实验,观察荷瘤动物的生长情况和肿瘤生长曲线,测定肿瘤生长抑制率;肿瘤组织病理学切片进行HE染色形态学观察,其超微结构行透射电镜观察。结果苦参碱对小鼠的H22实体瘤生长具有明显的抑制作用,抑瘤率达60%以上。苦参碱处理组小鼠的成瘤时间明显晚于生理盐水对照组,肿瘤生长也较对照组慢。组织学观察显示,肿瘤细胞大量变性坏死,电镜下可见凋亡细胞和凋亡小体。结论苦参碱具有较强的体内抗肿瘤作用,作用机制可能与其能够抑制肿瘤细胞的分裂和增殖、直接杀伤肿瘤细胞和(或)诱导肿瘤细胞凋亡、调节机体的抗肿瘤免疫应答效应有关。更多还原

【Abstract】 Objective To investigate the inhibitory effect of matrine on tumor growth in tumor bearing mice and explore its possible mechanisms of anti tumor action in vivo. Methods Hepatocellular carcinoma cells H 22 were subcutaneously injected into BALB/c mice and matrine was administered to the tumor bearing mice. The kinetics of tumor formation and tumor growth were measured, tumor growth inhibition rate (IR) was calculated, and tumor tissue samples were taken and examined by light and electron microscopy to assess the inhibitory effects of matrine on tumor growth in the mice. Results Marked inhibitory effect of matrine on the transplanted hepatocellular carcinoma H 22 was observed in the tumor bearing mice. The inhibitory rates were 62.5% and 60.7% in the groups treated with high and low dosage of matrine,respectively ( P <0.01 vs. control group). The tumor formation was significantly retarded and tumor growth was inhibited in matrine treated groups compared with those in control mice. Histopathological examination revealed widespread necrosis with massive accumulation of infiltrating lymphocytes and plasmacytes in the tumors. Numerous apoptotic cells and apoptotic bodies were observed in the tumors under the electron microscope. Conclusion Matrine has marked inhibitory effects on tumor growth in vivo, which is probably related to inhibition of cell division and tumor cell proliferation, directly killing of tumor cells and/or induction of apoptosis and modulation of anti tumor immune responses.更多还原