【摘要】 目的探讨三氧化二砷(As2O3)诱导人宫颈癌HeLa细胞凋亡时,对HPV18E6致瘤基因和端粒酶活性的影响。方法用不同浓度的As2O3作用于HeLa细胞不同时间段后,光镜和电镜观察细胞形态改变,流式细胞仪检测细胞周期改变,MTT法测定细胞生长抑制情况。RT PCR方法检测HPV18E6的表达,TRAP ELISA方法测定细胞内端粒酶活性变化。结果2μmol/LAs2O3处理HeLa细胞48h后,可降低细胞存活率,诱导细胞凋亡,抑制HPV18E6的表达,细胞内端粒酶活性明显下降。As2O3对HeLa细胞的这种作用呈时间剂量依赖关系。结论As2O3诱导HeLa细胞凋亡可能与其抑制HPV18E6致瘤基因的表达有关,细胞内端粒酶活性的抑制可能与这种E6致瘤基因的抑制有关。更多还原

【Abstract】 Objective To study the effect of As₂O₃ induction of HeLa cell apoptosis on HPV18 E6 expression and telomerase activity. Methods HeLa cells were treated with As₂O₃ in various concentrations. The effect of As₂O₃ on HeLa cell survival and apoptosis was determined by MTT assay, light and electron microscopy, and flow cytometry. Telomerase activity in HeLa cells was determined by TRAP-ELISA and the expression of HPV18 E6 mRNA was assayed by RT-PCR. Results After being treated with 2 μmol/L As₂O₃ for 48 h, the survival of HeLa cells decreased, and marked apoptosis was observed in a time-and dose-dependent manner. There was a good correlation between cell apoptosis and viral E6 gene expression and inhibition of telomerase activity following As₂O₃ treatment. Conclusion The molecular mechanisms of As₂O₃ effect on HeLa cells may be related to down-regulation of HPV18 E6 oncogene expression and inhibition of telomerase activity.更多还原