【摘要】 目的了解耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)产酶耐药基因流行病学状况。方法我们对55 株MRCNS菌进行了TEM 1基因(编码青霉素酶)、aph(3’) Ⅲ和aac(6’)/aph(2")基因(编码氨基糖苷类修饰酶)、erm基因(编码红霉素甲基化酶)检测。结果55 株MRCNS菌TEM 1、aph(3’) Ⅲ和aac(6’)/aph(2")、erm基因阳性株分别为54、43、16、45 株,阳性率分别为98 .2%、78 .2%、29 .1%、81. 8%;其中同时检出TEM -1 基因、aph(3’) Ⅲ或aac(6’)/aph(2")基因、erm基因39 株(70 .9%)。结论70 .9% MRCNS菌已同时携带青霉素酶、氨基糖苷类修饰酶和红霉素甲基化酶基因。更多还原

【Abstract】 OBJECTIVE To study the epidemiology of drug-resistant genes of productive enzyme in meticillin-resistant coagulase negative staphylococcus(MRCNS). METHODS TEM-1 gene of the β-lactamase, aph(3′) -Ⅲand aac(6’)/aph(2") genes of aminoglycoside-modifying enzyme(AME) and erm gene of erythromycin methyltransferase were detected by polymerase chain reaction (PCR) in 55 MRCNS strains. RESULTS TEM-1 gene,aph(3’)-Ⅲ gene, aac(6’)/aph(2") gene and erm gene were detected in 54,43,16 and 45 strains, respectively. From 55 MRCNS strains. The positive rate of genes was higher than 70% that β-lactamase and aminoglycoside-modifying enzyme(AME) and erythromycin methyltransferase in MRCNS. CONCLUSIONS The drug-resistant rate is higher in MRCNS. Three to 4 drug-resistant genes are obtained by the most drug-resistant bacteria at the same time.更多还原