【摘要】 目的采用体外细胞培养方法,研究不同浓度c9,t11共轭亚油酸(c9,t11CLA)对人胃腺癌细胞(SGC7901)中亚油酸代谢途径的限速酶的影响。方法用200、100、50和25μmol/L浓度的c9,t11CLA处理SGC7901细胞24h,四甲基偶氮唑盐实验检测c9,t11CLA对SGC7901细胞增殖的抑制作用,采用逆转录聚合酶链反应检测c9,t11CLA对SGC7901细胞中亚油酸代谢途径的Δ6脱氢酶、Δ5脱氢酶、环氧合酶(COX)1、COX2和5脂氧合酶(5LOX)mRNA表达的影响。结果在200、100、50和25μmol/L浓度时,c9,t11CLA对SGC7901增殖的抑制率分别为54.3%、20.5%、10.5%、2.93%;均可下调COX2mRNA的表达,上调Δ6脱氢酶、COX1mRNA的表达,但对Δ5脱氢酶和5LOXmRNA表达的影响不显著。结论c9,t11CLA可通过调节Δ6脱氢酶和COX的表达抑制肿瘤细胞的增殖,说明c9,t11CLA通过影响亚油酸代谢途径中限速酶的基因表达而改变类二十碳烷酸的形成,推测c9,t11CLA影响肿瘤细胞中亚油酸代谢途径的限速酶是其发挥抗癌活性的另一作用机制。更多还原

【Abstract】 Objectives To study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell(SGC-7901).Methods SGC-7901 was treated with c9,t11-CLA by 200,100,50 or 25μmol/L for 24 hours.The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Δ6-desaturase,Δ5-desaturase,COX-1,COX-2,5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).Results At a concentration of 200,100,50,or 25μmol/L,c9,t11-CLA suppressed the proliferation of SGC-7901 by 54.3%,20.5%,10.5% and 2.93%. The c9,t11-CLA might decrease the expression of COX-2 mRNA ,and increase the expression of Δ6-desaturase and COX-1 in SGC-7901, but might not affect Δ5-desaturase and 5-LOX.Conclusion The effects of c9,t11-CLA on the COX and Δ6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anti-cancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.更多还原