【摘要】 目的研究饮食诱导肥胖大鼠胰岛素抵抗形成过程中脂肪组织抵抗素基因的表达水平和共轭亚油酸(CLA)对其的影响,探讨抵抗素的作用及其与过氧化物酶体增殖物活性受体γ(PPARγ)之间的关系。方法选用雄性Wistar大鼠,分为对照组、高脂组、高脂+CLA组(每100g饲料含CLA分别为0.75g、1.50g、3.00g),每组10只大鼠,观察CLA对肥胖大鼠胰岛素和血糖水平的影响,并应用逆转录聚合酶链反应检测抵抗素和PPARγ的表达水平。结果高脂组大鼠血清胰岛素和血糖水平分别为(11.11±2.73)mIU/L和(5.09±0.66)mmol/L,CLA可降低肥胖大鼠的血清胰岛素和血糖水平,低、中、高剂量组胰岛素水平分别为(6.99±1.77)mIU/L,(7.36±1.48)mIU/L,(7.85±1.60)mIU/L,血糖水平分别为(4.28±0.72)mmol/L、(4.18±0.55)mmol/L、(4.06±0.63)mmol/L,且高脂组大鼠脂肪组织抵抗素、PPARγmRNA的表达较基础组增强,CLA可增加肥胖大鼠脂肪组织抵抗素、PPARγmRNA的表达水平。结论肥胖大鼠脂肪组织抵抗素mRNA表达较基础组增加,CLA可通过激活PPARγ上调抵抗素基因的表达,从而改善胰岛素抵抗。更多还原

【Abstract】 Objective To study the gene expression of the resistin and the effects of conjugated linoleic acid on its expression in white adipose tissue of obese rats fed with high fat diet during the formation of insulin resistance.Methods Male Wistar rats were randomly separated in control group, high-fat group and high fat + conjugated linoleic acid(CLA) group (0.75 g,1.50 g,3.00 g per 100 g diet weight), using reverse transcription polymerse chain reaction (RT-PCR) technique to measure the expression level of resistin and peroxisome proliferator-activated receptor-γ(PPARγ) mRNA expression.Results the serum insulin and glucose levels of obese rats were (11.11±2.73) mIU/L, (5.09±0.66) mmol/L, and supplement of CLA might decrease hyperinsulinemia and hyperglycemia, in CLA group(0.75 g,1.50 g, 3.00 g per 100 g diet weight) the serum insulin levels were( 6.99±1.77) mIU/L,(7.36±1.48) mIU/L,(7.85±1.60) mIU/L, and glucose levels were (4.28±0.72) mmol/L,(4.18±0.55) mmol/L,(4.06±0.63) mmol/L. The expression of resistin in adipose tissue of obese rat fed with high fat diet was increased as compared with those fed with basic diet. CLA might increase the expression of resistin and PPARγ in adipose tissue of obese rat.Conclusion The expression of resistin mRNA of obese rat fed with high fat diet was higher than those fed with basic diet,and CLA might improve the insulin resistance in obese rats and possibly upregulate the expression of resistin through activing PPARγ.更多还原