【摘要】 目的研究共轭亚油酸对饮食诱导肥胖大鼠脂联素基因表达的影响。方法选用雄性Wistar大鼠,随机分为对照组、高脂组、高脂+共轭亚油酸组(每100g饲料含共轭亚油酸分别为075g、150g、300g),每组动物10只,观察共轭亚油酸对肥胖大鼠胰岛素、血糖水平的影响,并应用RTPCR的方法检测脂联素、过氧化物酶体增殖物活性受体γ(PPARγ)的表达水平。结果高脂组大鼠血清胰岛素和血糖水平分别为(1111±273)μIU/ml,(509±066)mmol/L,075%、150%、300%剂量组胰岛素水平分别为(699±177)μIU/ml,(736±148)μIU/ml,(785±160)μIU/ml,血糖水平分别为(428±072)mmol/L,(418±055)mmol/L,(406±063)mmol/L,且共轭亚油酸可增加肥胖大鼠脂肪组织脂联素、PPARγmRNA的表达水平。结论共轭亚油酸可通过激活PPARγ上调脂联素基因的表达,改善肥胖大鼠的胰岛素抵抗。更多还原

【Abstract】 Objective To study the effect of conjugated linoleic acid (CLA) on expression of adiponectin in white adipose tissue of obese rats.Methods Male Wistar rats were randomly divided into control group, high-fat group and high fat + CLA group (0.75 g,1.50 g,3.00 g per hundred gram diet weight ), we observed the effect of CLA on serum insulin and glucose levels of obese rats,and the reverse transcription polymerse chain reaction (RT-PCR) technique was used to measure the expression level of adiponectin and peroxisome proliferator-activated receptor-γ (PPARγ)mRNA .Results The serum insulin and glucose levels of obese rats were (11.11±2.73)μIU/ml, (5.09±0.66)mmol/L.The supplement of CLA decreased the hyperinsulinemia and hyperglycemia , the serum insulin in CLA group(0.75 g,1.50 g,3.00 g per hundred gram diet weight) were( 6.99±1.77)μIU/ml,(7.36±1.48)μIU/ml,(7.85±1.60)μIU/ml(P<0.05),and glucose were (4.28±0.72)mmol/L,(4.18±0.55)mmol/L(P<0.05),(4.06±0.63)mmol/L(P<0.05),CLA can increase the expression of adiponectin and PPARγ in adipose tissue of obese rat.Conclusion The CLA might improve the insulin resistance of the obese rat and increase the expression of adiponectin mRNA, which mightpossibly act through activating PPARγ.更多还原