【摘要】 目的检测和分析沈阳汉族HLA-A、-B、-Cw位点等位基因的多态性。方法采用聚合酶链反应-寡核苷酸探针杂交分型方法对108名沈阳籍健康献血员进行HLA-A、-B、-Cw等位基因分型。结果检出等位基因HLA-A位点21个,B位点43个,Cw位点23个;所有位点的等位基因频率分布符合Hardy-Weinberg平衡。结论从基因水平分析了HLAⅠ类基因的群体分布特征,提供了沈阳汉族群体HLAⅠ类基因座更准确的基因频率。更多还原

【Abstract】 Objective To investigate, at the DNA level, the polymorphism of HLA-A,-B,-Cw genes in the Chinese of Han ethnicity in Shenyang. Methods Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A,-B and-Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area. Results The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium. Conclusion Using molecular method, the present authors have analyzed the characteristic of HLA Ⅰ distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.更多还原