【摘要】 目的探讨重组人(rh)IL-11、rhG-CSF联合应用对小鼠脾脏T淋巴细胞数量和功能的影响及其机制。方法应用流式细胞仪对细胞因子处理后的各组T淋巴细胞和亚群、共刺激分子CD28以及抑制性T淋巴细胞(CD3+CD4-CD8-、CD8+CD28-、CD4+CD25+)的数量进行测定;应用MTT法检测各组T淋巴细胞的增殖能力、混合淋巴细胞反应;测定细胞内IL-4、IFN-γ的分泌情况。结果经rhG-CSF单独及rhIL-11、rhG-CSF联合处理淋巴细胞总数和T细胞亚群与对照组比较均下降(P<0.05),而联合处理组CD3+、CD4+、CD8+细胞下降较明显,与其它各组比较差异有统计学意义(P<0.01),CD4+/CD8+细胞的比值联合处理组亦低于其它各组;G-CSF组及联合处理组CD3+CD28+细胞比例与对照组比较下降(P<0.01),CD4+CD28+、CD8+CD28+细胞联合处理组虽较其它各组低,但各组间差异无统计学意义;CD3+CD4-CD8-和CD4+CD25+抑制性T淋巴细胞各组间均无明显差异,而联合处理组CD8+CD28-抑制性T淋巴细胞较其它各组增加(P<0.05);细胞因子动员后各组T淋巴细胞增殖能力、供鼠对异种抗原的反应能力均降低(P<0.05),而联合处理组比其它各组下降更明显;联合处理组、G-CSF组与对照组比较细胞内细胞因子IFN-γ水平下降、IL-4水平升高,联合处理组与G-CSF组比较差异无统计学意义,但IFN-γ/IL-4的比值联合组低于其它各组(P<0.05)。结论rhIL-11与rhG-CSF体内应用后可产生协同作用,通过对T淋巴细胞数量和功能的影响来诱导机体免疫耐受。更多还原

【Abstract】 Objective To investigate the effect of in vivo administration of rhG-CSF and/or rhIL-11 on mice immune system function. Methods T cell subgroups, suppressor T cells （CD8⁺CD28^-, CD4⁺CD25⁺, CD3⁺CD4^-CD8^- T cells）, expression of CD28 on T cells, and spleen T cells intracellur IL-4/IFN-γ secretion were determined by multicolor flow cytometry. MTT was used to determine the T cell proliferation capacity and mixed lymphocyte reactions.Results In vivo administration of cytokines decreased the percentage of lymphocytes （P<0.05）, rhIL-11 and rhG-CSF in combination significantly decreased the CD4⁺/CD8⁺ ratio and increased the percentage of CD8⁺CD28^- suppressor T cells compared to either cytokine alone （P<0.01）. There was no difference in the percentage of CD3⁺CD4^-CD8^- and CD4⁺CD25⁺ suppressor T cells between either of the cytokines. Fourthermore, cytokines treatments significantly decreased the capacities of splenic T cells proliferation and the response to alloantigens compared with the PBS treatment （P<（0.05））, the combination group being more significantly decreased （P<0.01）. And cytokines treatment significantly decreased the production of IFN-γ and increased the production of IL-4 compared with the PBS treatment（P<0.05）.The ratio of IFN-γ/IL-4 were significantly decreased after the combination compared with either of them alone. Conclusion The combination of rhIL-11 and rhG-CSF is potentially synergistic in the induction of immune tolerance by their effects on the proliferation capacity and function of T lymphocytes.更多还原