【摘要】 目的了解天津地区汉坦病毒分离株TJJ16S基因的分子特征。方法采用细胞培养法从鼠肺标本中分离汉坦病毒,命名为TJJ16,提取TJJ16感染VeroE6细胞的总RNA,经逆转录扩增病毒cDNA,继而进行PCR扩增S基因片段,纯化后克隆于pMD18T载体,测定核苷酸序列并进行分析。结果天津地区首次从社鼠中分离出汉坦病毒TJJ16,其S基因片段全序列长为1701nt。只有1个编码N蛋白的开放读码框架(ORF),起始密码子为第37nt,终止于1326nt,推导其编码的蛋白长为429个氨基酸。经核苷酸系统发生树分析,TJJ16株应归属为汉坦病毒的汉滩型(HTN型)毒株,与HTN型新亚型A16株同属一个亚型。结论TJJ16病毒株的分离与S基因片段序列分析,证实在天津市鼠间存在HTN型汉坦病毒感染,对临床和流行病学的研究具有重要意义。更多还原

【Abstract】 Objective To study the molecular character of S gene segment from hantavirus strain TJJ16 isolated in Tianjin. Methods The hantavirus strain was isolated and total cellular RNA were extracted from Vero E6 cells infected with hantavirus. Viral S gene segment was amplified by reverse transcripase polymerase chain reaction (RT PCR) and cloned into pMD 18T vector. The S segment was sequenced. Results It is the first isolation of hantavirus from host Rattus confucianus in Tianjin. The S segment was 1701 nt in length; only one ORF (from 37 nt to 1326 nt) encoded 429 amino acids. Phylogenetic tree showed that TJJ16 strain belongs to HTN type virus, the same subtype as A16 strain. Conclusion HTNV infection was proved by the isolation and sequence of TJJ16 virus strain in Tianjin. It is very important for study on clinical research and epidemiology.更多还原